Journal: Cell Systems

Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis

doi: 10.1016/j.cels.2018.08.011

Figure Lengend Snippet:

Article Snippet: After 4 dpi, half of the mTeSR 1 media was replaced with supplemented BrainPhys media (05790, StemCell Technologies), replaced daily until 7 dpi and further once per week.

Techniques: Recombinant, Luciferase, cDNA Synthesis, Plasmid Preparation, Software

Journal: bioRxiv

Article Title: MAPT expression is regulated by long-range interactions with cis -regulatory elements

doi: 10.1101/2023.03.07.531520

Figure Lengend Snippet: A) Loop plot of HiCCUPs nominated loops to the MAPT promoter. Top panel: Nominated loops by dataset. Top: HiC loops to the MAPT promoter from iCell GlutaNeuron cultures. Middle: Capture-C loops to the MAPT promoter from iCell GlutaNeuron cultures. Bottom: Capture-C loops to the MAPT promoter from iCell GABANeuron cultures. Bottom panel: Top: Track of ENCODE annotated candidate CREs. Middle: ATAC-seq peaks from KOLF2.1J-hNGN2 differentiated neurons. Bottom: H3K27ac and CTCF ChIP-seq peaks from Day 14 BrainPhys differentiated neurons. B) Proportion of HiC global loop calls by ENCODE annotation. C) Proportion of Capture-C MAPT promoter loops by ENCODE annotation for both iCell GlutaNeurons and GABANeurons.

Article Snippet: On day 3 neurites were present, and cells were renewed with Cortical Neuron Culture Media: BrainPhys neuronal medium (StemCell Technologies), 50X SM1 supplement (StemCell Technologies), 10 μ g/mL BDNF (StemCell Technologies), 10 μ g/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco).

Techniques: Capture-C, ChIP-sequencing

Journal: bioRxiv

Article Title: MAPT expression is regulated by long-range interactions with cis -regulatory elements

doi: 10.1101/2023.03.07.531520

Figure Lengend Snippet: A) Heatmap showing expression of MAPT and cell type marker genes in NPCs, Day 14 BrainPhys differentiated neurons (BrainPhys Neurons), KOLF2.1J-hNGN2 Neurons (NGN2 Neuron), and iCell GlutaNeurons. B) Differential Capture-C directional p-value track. Top panel: Comparison of NPCs and Day 14 BrainPhys Neurons. Middle panel: Comparison of iCell GlutaNeurons (Excitatory) and iCell GABANeurons (Inhibitory). C) Zoom in of chr17: 45,225,000-45,320,000. Top panels: Chromatin accessibility across cell types generated by snATAC-seq in DLPFC nuclei. Bottom panels: Differential capture-C comparing BrainPhys differentiated neurons and NPCs with the neuron-specific region highlighted in gray.

Article Snippet: On day 3 neurites were present, and cells were renewed with Cortical Neuron Culture Media: BrainPhys neuronal medium (StemCell Technologies), 50X SM1 supplement (StemCell Technologies), 10 μ g/mL BDNF (StemCell Technologies), 10 μ g/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco).

Techniques: Expressing, Marker, Capture-C, Generated

Journal: bioRxiv

Article Title: MAPT expression is regulated by long-range interactions with cis -regulatory elements

doi: 10.1101/2023.03.07.531520

Figure Lengend Snippet: A) Boxplots showing elements tested in luciferase assays in iCell GlutaNeurons (*p<0.05, ANOVA with Fisher’s LSD). B) Boxplots showing elements tested in luciferase assays in KOLF2.1J-hNGN2 neurons (*p<0.05, ANOVA with Fisher’s LSD). C) RT-qPCR of MAPT expression. Barplots show regions tested in Day 14 BrainPhys differentiated neurons by CRISPRi experiments (*p<0,05, ANOVA with Fisher’s LSD).

Article Snippet: On day 3 neurites were present, and cells were renewed with Cortical Neuron Culture Media: BrainPhys neuronal medium (StemCell Technologies), 50X SM1 supplement (StemCell Technologies), 10 μ g/mL BDNF (StemCell Technologies), 10 μ g/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco).

Techniques: Luciferase, Quantitative RT-PCR, Expressing

Journal: bioRxiv

Article Title: MAPT expression is regulated by long-range interactions with cis -regulatory elements

doi: 10.1101/2023.03.07.531520

Figure Lengend Snippet: A) Boxplots showing statistically significant (*p<0.05, ANOVA with Fisher’s LSD) elements tested in luciferase assays in iCell GlutaNeurons. B) Boxplots showing statistically significant (*p<0.05, ANOVA with Fisher’s LSD) elements tested in luciferase assays in KOLF2.1J-hNGN2 neurons. C) RT-qPCR of MAPT expression. Barplots show statistically significant regions tested in Day 14 BrainPhys differentiated neurons by CRISPRi experiments. Red asterisk indicates regions significant by 3’ mRNA-seq and RT-qPCR. Gray asterisk indicates regions significant in either RT-qPCR or 3’ mRNA-seq. (*p<0.05, ANOVA with Fisher’s LSD). D) Heatmap showing differential expression of MAPT and all genes expressed (cpm > 30) within 1 Mb of MAPT TSS. Left y-axis indicates the distance of the midpoint of the target region tested in CRISPRi experiments from the MAPT promoter (chr17:45894000). textbfE) Browser view of significant nominated regions. Green: iCell GlutaNeuron luciferase assay. Blue: KOLF2.1J-hNGN2 neuron luciferase assay. Teal: CRISPRi. Red: significant in luciferase and CRISPRi.

Article Snippet: On day 3 neurites were present, and cells were renewed with Cortical Neuron Culture Media: BrainPhys neuronal medium (StemCell Technologies), 50X SM1 supplement (StemCell Technologies), 10 μ g/mL BDNF (StemCell Technologies), 10 μ g/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco).

Techniques: Luciferase, Quantitative RT-PCR, Expressing

Journal: bioRxiv

Article Title: MAPT expression is regulated by long-range interactions with cis -regulatory elements

doi: 10.1101/2023.03.07.531520

Figure Lengend Snippet: Zoom in on regions validated by qPCR and 3’ mRNA-seq ( A) −652,338, B) −464,677 I&-461,949, C) −44,905, D) 48,416). Top panel: H3K27ac signal from Day14 BrainPhys Neurons. Middle panel: Normalized Accessibility from KOLF2.1J-hNGN2 differentiated Neurons. Bottom panel: Tracks of design region, luciferase region (Green: tested in iCell Gluta Neurons; Blue: tested in KOLF2.1J-hNGN2 neurons), gRNA position, and nominated regions (DLPFC Links: Blue: control-specific link; gray: common link; Red: AD-specific link).

Article Snippet: On day 3 neurites were present, and cells were renewed with Cortical Neuron Culture Media: BrainPhys neuronal medium (StemCell Technologies), 50X SM1 supplement (StemCell Technologies), 10 μ g/mL BDNF (StemCell Technologies), 10 μ g/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco).

Techniques: Luciferase

Journal: Scientific Reports

Article Title: An organ-on-chip device with integrated charge sensors and recording microelectrodes

doi: 10.1038/s41598-023-34786-5

Figure Lengend Snippet: Biocompatibility and electrophysiological proof of concept experiments: ( a , b ) HiPSC-derived cortical neurons differentiated (7 days) and matured (14 days) on-chip. After staining, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta 3$$\end{document} β 3 tubulin-positive neurons (green) and synaptic vesicles (red) are visible. ( c ) Compatibility with Multichannel Systems MEA recording setup of the PCB and the chip. ( d ) Inset showing the chip and the well with medium and cells. ( e ) Single-electrode trace signal from the MEA recordings. ( f ) The addition of Picrotoxin (50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu \hbox {M}$$\end{document} μ M ) caused an increase in fire rate.

Article Snippet: Lastly, the hiPSC-derived cortical neurons were matured and maintained in BrainPhys hiPSC Neuron kit media (05795, StemCell Technologies) for the remainder of the experiment.

Techniques: Derivative Assay, Staining